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Product name
SR3300 Microscope Stain
Alternative names
CFW, FBA 28, FBA 113, Photine CU Liquid, Calcofluor 2MR
Description
SR3300 is Renaissance Chemicals Ltd liquid calcofluor type microscope stain. This liquid fluorophor microscope stain is used by microscopists for the study of cellulosic specimens, and is sold to universities and research organisations.
SR3300 calcofluor type microscope stain is available to universities and research establishments world wide, and in Asia we have distributors in Korea, Japan, Taiwan.and China
Distributors
Korea https://www.usbio.co.kr/
Japan https://en.tokyofuturestyle.com/
Taiwan https://tw.tokyofuturestyle.com/
China http://www.sha-jingyang.com/
SR3300 is also a fluorchrome, and is used for the rapid detection of yeasts, fungi, and parasitic organisms. It is a non-specific stain that binds to cellulose and chitin in cell walls, and is a stain for plant cells, similar to calcofluor. However, calcofluor stains are more prone to crystallisation than SR2200. Nevertheless, calcofluor stains are widely used and SR3300 is calcofluor type. 1g/l of SR3300 can be combined with 0.5g/L of Evans Blue to improve contrast.
Historical Information
Calcofluor was the trade name of American Cyanamid, the original manufacturer. It was an FBA 28 optical brightening agent, and also became a microscope stain. After they ceased production Hickson and Welch Ltd, Castleford, England sold their FBA 28 optical brightening agent to laboratory suppliers who rebranded Hickson’s Photine CU Liquid as calcofluor.
SR3300 is also FBA 28 Liquid optical brightening agent with the same composition as Photine CU Liquid. Hickson and Welch closed in 2005 and the founder of Renaissance Chemicals Ltd is a former Hickson and Welch employee and fluorescer specialist.
American Cyanamid Company was founded in 1907 by civil engineer Frank Washburn. It began as a pioneer in fertiliser manufacturing utilising calcium cyanamide. The trade name Calcofluor survived the demise of Cyanamid.
Footnotes
Smart and Laidlaw reported on Hickson and Welch’s Photine CU Liquid as a water tracer.
Renaissance Chemicals Ltd sells many types of fluorescent chemicals and fluorescent dyes, including near infra red fluorescers.
Application
Microscope Stain, Water Tracer
Quantity
50G
Package
Bottle
Availability
In Stock
Price
£40.00, €46.00, $53.00 Delivery extra
Other information
Prices
UK
£40 +£6.00 Delivery. If you need large quantities ask us for trade prices.
European Union, Switzerland, Norway
€46 + €16 Delivery
Rest of the World
$53.00 + $40.00 Delivery
We Accept Card Payments
Further reading & references
Below you’ll find a curated collection of references related to this product. These sources include academic research, review articles, and case studies that explore the chemistry, applications, and sustainability benefits our products. Whether you’re looking for in-depth technical details or broader insights into their real-world use, these references are a great place to start.
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An evaluation of some fluorescent dyes for water tracing
Smart and Laidlaw. 1977. Water Research.
https://doi.org/10.1029/WR013i001p00015 -
Water tracing in tropical regions, the use of fluorometric techniques in Jamaica
Smart and Laidlaw. Journal of Hydrology.
https://www.sciencedirect.com/science/article/abs/pii/0022169476900974 -
A comparison of the highly selective fluorescence staining of fungi in tissue sections with Uvitex 2B and Calcofluor White M2R
E.D. Wachsmuth. April 1988. Histochem J . 1988 Apr;20(4):215-21.
https://pubmed.ncbi.nlm.nih.gov/3061981/
Product number
31SR330025
CAS Number
4193-55-9
EC Number
224-073-5
